Packet M: Bonus 16
CASY technology is used to count these entities without the need for an electrolyte reservoir, unlike a similar apparatus invented by Walter Coulter. For 10 points each:
[10m] Name these entities that are loaded into a Neubauer counting chamber etched with five different size grids. The viability of these entities can be determined by using propidium iodide or Trypan blue staining.
ANSWER: cells [accept cytes; accept dead cells or apoptotic cells or necrotic cells; accept cell counting or cell viability assays]
[10e] Metabolic cell viability assays often quantify the amount of this molecule produced, such as after adding a firefly luciferase. This molecule is mostly generated by a namesake synthase in the inner mitochondrial membrane.
ANSWER: ATP [or adenosine triphosphate]
[10h] Another common colorimetric viability assay uses this dye, known by a three-letter acronym, which is converted into the purple compound formazan by cellular oxidoreductases.
ANSWER: MTT [or dimethylthiazol diphenyltetrazolium bromide; accept MTT assay or MTT cell viability assay; accept MTS or dimethylthiazol carboxymethoxyphenyl sulfophenyl tetrazolium; prompt on tetrazoles or tetrazolium salts]
<Ohio State A, Biology> | Packet M - Columbia A, Ohio State A, Maryland A, Toronto C, Clemson A, Notre Dame A
| Heard | PPB | E % | M % | H % |
|---|---|---|---|---|
| 49 | 11.84 | 92% | 27% | 0% |
Conversion
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
|---|---|---|---|---|---|---|
| Liberty A | North Carolina C | 0 | 10 | 0 | 10 | E |
| Liberty C | North Carolina A | 0 | 10 | 0 | 10 | E |
| NC State B | Liberty B | 0 | 10 | 0 | 10 | E |
| South Carolina A | VCU | 10 | 10 | 0 | 20 | ME |
| South Carolina B | North Carolina B | 10 | 10 | 0 | 20 | ME |
| Virginia | Virginia Tech A | 0 | 10 | 0 | 10 | E |
| William and Mary | Virginia Tech B | 10 | 10 | 0 | 20 | ME |
Summary
| Tournament | Edition | Match | Heard | PPB | E % | M % | H % |
|---|---|---|---|---|---|---|---|
| California (South) | Main Site | ✓ | 4 | 10.00 | 75% | 25% | 0% |
| Canada | Main Site | ✓ | 10 | 12.00 | 90% | 30% | 0% |
| Lower Mid-Atlantic | Main Site | ✓ | 1 | 10.00 | 100% | 0% | 0% |
| Lower Mid-Atlantic | Main Site | ✓ | 6 | 15.00 | 100% | 50% | 0% |
| Midwest | Main Site | ✓ | 8 | 16.25 | 100% | 63% | 0% |
| Northeast | Main Site | ✓ | 5 | 10.00 | 80% | 20% | 0% |
| Overflow | Main Site | ✓ | 3 | 10.00 | 100% | 0% | 0% |
| UK | Main Site | ✓ | 12 | 9.17 | 92% | 0% | 0% |