Packet L: Bonus 6
This technique is used to separate lipids, proteins, and nucleic acids from cell lysates based on the immiscibility between phenol and chloroform. For 10 points each:
[10m] Name this technique whose efficiency is quantified by the partition coefficient, the concentration ratio between two liquid phases. This technique is often done by shaking a cone-shaped glass piece capped by a stopper.
ANSWER: liquid-liquid extraction [accept LLE; accept phenol-chloroform extraction]
[10h] Following extraction, proteins can also be “salted out” by adding this kosmotropic compound. Electrolysis of this salt produces a related compound that is added to TEMED and acrylamide (“uh-KRILL-uh-myde”) to prepare gels for SDS-PAGE (“S-D-S-page”).
ANSWER: ammonium sulfate [or (NH4)2SO4; accept ammonium persulfate or APS; prompt on sulfates; reject “ammonia sulfate”]
[10e] Insoluble macromolecules are often pelleted during this process before they are resuspended and extracted. Soluble substances remain in the supernatant after this process, which is usually performed at thousands of RPMs.
ANSWER: centrifugation [or centrifuge or centrifuging; accept microcentrifugation or ultracentrifugation; accept spinning or spin down; prompt on rotation; prompt on sedimentation; prompt on settling]
<AL, Chemistry> | Packet L - Harvard A, McGill A, Arizona State A, Georgia Tech B, Toronto D, Georgia Tech D
| Heard | PPB | E % | M % | H % |
|---|---|---|---|---|
| 51 | 13.92 | 98% | 37% | 4% |
Conversion
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
|---|---|---|---|---|---|---|
| Catholic | NYU C | 0 | 0 | 10 | 10 | E |
| Georgetown | Rutgers A | 0 | 0 | 10 | 10 | E |
| John Jay | Vassar | 0 | 0 | 10 | 10 | E |
| Johns Hopkins B | Maryland B | 0 | 0 | 10 | 10 | E |
| NYU A | Johns Hopkins A | 0 | 0 | 10 | 10 | E |
| NYU B | Maryland A | 0 | 0 | 10 | 10 | E |
Summary
| Tournament | Edition | Match | Heard | PPB | E % | M % | H % |
|---|---|---|---|---|---|---|---|
| Great Lakes | Main Site | ✓ | 5 | 16.00 | 100% | 60% | 0% |
| Lower Mid-Atlantic | Main Site | ✓ | 7 | 11.43 | 86% | 29% | 0% |
| Lower Mid-Atlantic | Main Site | ✓ | 1 | 10.00 | 100% | 0% | 0% |
| Midwest | Main Site | ✓ | 9 | 16.67 | 100% | 56% | 11% |
| Northeast | Main Site | ✓ | 5 | 18.00 | 100% | 60% | 20% |
| Southeast | Main Site | ✓ | 6 | 15.00 | 100% | 50% | 0% |
| UK | Main Site | ✓ | 12 | 12.50 | 100% | 25% | 0% |
| Upper Mid-Atlantic | Main Site | ✓ | 6 | 10.00 | 100% | 0% | 0% |