Packet G: Tossup 17

A key step of the “SeSaM” version of this technique is strand cleavage at phosphorothioate (“phosphor-oh-thio-ate”) groups followed by amplification with TdT. The “insertional” form of this technique uses P-element or Sleeping Beauty transposons. (10[1]-5[1])One form of this technique transforms DUT and UDG deficient E. coli strains with (-5[1])M13 phagemids (10[1])and was developed by Thomas Kunkel. Alanine scanning is a diagnostic form of this technique, (10[1])which can be done randomly (10[1])using ethyl nitrosoureas (“nitroso-ureas”). A form of this technique uses a variant of PCR (-5[1])with manganese (-5[1])ions (10[1]-5[1])instead of magnesium (10[1]-5[1])ones (-5[1])to coordinate (10[1])“sloppy” Taq polymerases. (10[1])Stratagene developed (-5[1])the “Quikchange” kit for the “site-directed” (10[2])form (10[2])of (10[1])this (10[3])technique. (10[10]-5[1])For 10 points, (10[2])name this technique for introducing sequence (-5[1])changes (10[2])into (-5[1])a target (-5[2])genome. ■END■ (10[3]0[73])

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