Round 5: Tossup 17
A key step of the “SeSaM” version of this technique is strand cleavage at phosphorothioate (“phosphor-oh-thio-ate”) groups followed by amplification with TdT. The “insertional” form of this technique uses P-element or Sleeping Beauty transposons. One form of this technique transforms DUT and UDG deficient E. coli strains with M13 phagemids and was developed by Thomas Kunkel. Alanine scanning is a diagnostic form of this technique, which can be done randomly using ethyl nitrosoureas (“nitroso-ureas”). A form of this technique uses a variant of PCR with manganese ions instead of magnesium ones to coordinate “sloppy” Taq polymerases. Stratagene developed the “Quikchange” kit for the “site-directed” form of this technique. For 10 points, name this technique for introducing sequence changes into a target genome. ■END■
ANSWER: mutagenesis [accept saturation or insertional or random or site-directed mutagenesis; accept answers involving making mutations; accept error-prone PCR or epPCR until read; prompt on polymerase chain reaction or PCR until read by asking “what is that being used to accomplish?”; prompt on directed evolution by asking “what technique is used to drive that?”] (The first sentence refers to sequence saturation mutagenesis.)
<AL, Biology> | Packet G - McMaster A, Notre Dame C, Rutgers A, NYU A
= Average correct buzzpoint
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