Round 5: Tossup 17

A key step of the “SeSaM” version of this technique is strand cleavage at phosphorothioate (“phosphor-oh-thio-ate”) groups followed by amplification with TdT. The “insertional” form of this technique uses P-element or Sleeping Beauty transposons. (-5[1])One form of this technique transforms DUT and UDG deficient E. coli strains with M13 phagemids (10[1])and was developed by Thomas Kunkel. Alanine scanning is a diagnostic form of this technique, which can be done randomly using ethyl nitrosoureas (“nitroso-ureas”). A form of this technique uses a variant of PCR with manganese ions instead of magnesium ones to coordinate “sloppy” Taq polymerases. Stratagene developed (-5[1])the “Quikchange” kit for the “site-directed” form of this technique. (10[2])For 10 points, name this technique for introducing sequence changes (10[1])into a target genome. ■END■ (10[1]0[8])

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